Spinal Cord Connections


Group Members

Brett Graham – Group Leader
Sally Dickinson – Research Assistant
Jamie Flynn – PhD Candidate
Mark Gradwell – PhD Candidate
Jessica Madden – Research Assistant
Kelly Smith – PhD Candidate

 

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The spinal cord plays a similar role to a telephone exchange

Research Focus
The spinal cord is much like a telephone exchange, receiving information from a multitude of channels, which must be preserved and processed before they can be directed to appropriate destinations. We know that in spinal cord injury those lines of communication are severed, halting the transmission of vital information and causing a loss of sensation and movement below the injury site. In chronic pain, these communication lines can become crossed and information is redirected to inappropriate destinations with the potential to make a gentle touch cause excruciating pain. Similarly, many movement disorders can be likened to a situation where communication lines are either crossed or broken with the consequence being a loss of smooth, efficient, coordinated movement.

In our efforts to understand and treat this range of spinally-based conditions, knowledge about how the lines of communication in this region are connected normally is critical if we are to repair and rewire them after damage. This is a task that has long been considered too immense given the sheer number of different nerve cell types that are interconnected in spinal cord networks, and the lack of anatomical organisation – ie, unlike a telephone exchange where wires and cables are organised in a roughly ordered manner, the connections of the spinal cord are intermingled in a chaotic and disorganised mosaic. Fortunately, a number of recent scientific breakthroughs have now given us tools to understand how spinal networks are connected and disconnected by disease and injury.

EM-Islet

The recovered morphology of a calretinin positive dorsal horn neuron. The white processes are dendrites, which receive information from other populations of neurons. The red processes are the axon, which conveys information from the pictured neuron to other nerve cell populations.

 

LASU imageOur laboratory includes a brain tissue slicing station, two state of the art in vitro electrophysiology setups, a dedicated laser stimulating and uncaging (LASU) setup, an in vivo electrophysiology setup, and a behavioural testing facility for assessing sensory thresholds and pain in rodents.  Experiments span from single channel analysis of individual receptor properties, to synaptic and intrinsic membrane properties of neurons using in vitro CNS slice preparations, and also whole animal in vivo studies of single neuron properties, and responses to natural stimuli.

Projects

1. The role of Hypothalamic Orexin circuits in neuropathic pain
2. The effect of ageing on spinal pain circuits
3. Molecular/genetic analysis of spinal circuits
4. Characterisation of Thy1 positive neurons in the dorsal horn
5. Characterisation of calretinin positive neurons in the deep dorsal horn of the sacral spinal cord

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